Simultaneous Determination of Xanthopterin and Isoxanthopterin in Human Urine by Synchronous Fluorescence Spectroscopy |
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Authors: | Yi-Qun Wan Li-Juan Tang and Ting Tan |
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Institution: | (1) State key Laboratory of Food Science and Technology, Nanchang University, Nanchang, Jiangxi, 330047, People’s Republic of China;(2) Center of Analysis and Testing, Nanchang University, Nanchang, Jiangxi, 330047, People’s Republic of China |
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Abstract: | A simple, rapid, sensitive and selective method for simultaneously determining xanthopterin and isoxanthopterin content in
human urine has been developed using synchronous fluorescence spectroscopy based on their intrinsic fluorescence. The synchronous
fluorescence spectra were obtained with Δλ = 65 nm in a pH 8.5 KH2PO4-NaOH buffer solution. The detected wavelengths of quantitative analysis were set at 410 nm for xanthopterin and 325 nm for
isoxanthopterin, respectively. Pretreatment of urine samples only was filtrated through a 0.45 μm membrane filter, which was
free from the tedious separation procedures. Under optimized conditions, the limits of detection (LOD) were 0.94 ng/mL for
xanthopterin and 0.48 ng/mL for isoxanthopterin. The recoveries ranged from 88.0% to 103.8 % for healthy and cancer urine
samples, with coefficient of variation between 2.09% and 7.06%. The proposed method has been successfully applied to the simultaneous
analysis for xanthopterin and isoxanthopterin in human urine. The results showed that the average level of isoxanthopterin
was significantly elevated in urine excreted by stomach cancer patients (P < 0.01), while no significant change of xanthopterin level was found between stomach cancer patients and healthy individuals.
This potentially indicates that an increase in amounts of isoxanthopterin can be associated with the presence of stomach cancer. |
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Keywords: | |
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