| A Novel Ratiometric Fluorescent Probe for Highly Sensitive and Selective Detection of β-Galactosidase in Living Cells |
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| 作者姓名： | Min Chen Lixuan Mu Xingxing Cao Guangwei She Wensheng Shi |
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| 作者单位： | Key Laboratory of Photochemical Conversion and Optoelectronic Materials;University of Chinese Academy of Sciences |
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| 基金项目： | National Key R&D Program of China(2016YFA0200800);NSFC(51672284);Beijing Natural Science Foundation(2181002);Chinese Academy of Sciences(1A1111KYSB20180017,QYZDJ-SSW-JSC032,XDB17000000). |
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| 摘 要： | β-Galactosidase,a glycoside hydrolase enzyme,has been proved to be an important biomarker of cell senescence and primary ovarian cancer.Effective detection of β-galactosidase has attracted wide attention.Herein,one ratiometric fluorescent probe has been successfully synthesized for detecting the β-galactosidase in living cells.The as-prepared probe exhibits two emission peaks at 490 nm and 530 nm,respectively,and the ratio of fluorescence intensities from the two emission peaks could be utilized to monitor the β-galactosidase.This present ratiometric fluorescent probe is,therefore,very promising for effective,sensitive,and selective detection of the β-galactosidase in living cells.
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| 关 键 词： | HAS BE |
A Novel Ratiometric Fluorescent Probe for Highly Sensitive and Selective Detection of β‐Galactosidase in Living Cells |
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| Min Chen,Lixuan Mu,Xingxing Cao,Guangwei She,Wensheng Shi.A Novel Ratiometric Fluorescent Probe for Highly Sensitive and Selective Detection of β‐Galactosidase in Living Cells[J].Chinese Journal of Chemistry,2019,37(4):330-336. |
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| Authors: | Min Chen Lixuan Mu Xingxing Cao Guangwei She Wensheng Shi |
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| Abstract: | β‐Galactosidase, a glycoside hydrolase enzyme, has been proved to be an important biomarker of cell senescence and primary ovarian cancer. Effective detection of β‐galactosidase has attracted wide attention. Herein, one ratiometric fluorescent probe has been successfully synthesized for detecting the β‐galactosidase in living cells. The as‐prepared probe exhibits two emission peaks at 490 nm and 530 nm, respectively, and the ratio of fluorescence intensities from the two emission peaks could be utilized to monitor the β‐galactosidase. This present ratiometric fluorescent probe is, therefore, very promising for effective, sensitive, and selective detection of the β‐galactosidase in living cells. |
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