Simultaneous determination of spinosad,temephos, and piperonyl butoxide in animal‐derived foods using LC–MS/MS |
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Authors: | Weijia Zheng Jeong‐Min Choi A M Abd El‐Aty Kyung‐Hee Yoo Da‐Hee Park Seong‐Kwan Kim Young‐Sun Kang Ahmet Hacmüftüolu Jing Wang Jae‐Han Shim Ho‐Chul Shin |
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Institution: | Weijia Zheng,Jeong‐Min Choi,A. M. Abd El‐Aty,Kyung‐Hee Yoo,Da‐Hee Park,Seong‐Kwan Kim,Young‐Sun Kang,Ahmet Hac?müftüo?lu,Jing Wang,Jae‐Han Shim,Ho‐Chul Shin |
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Abstract: | Pesticides, which are used as plant protection products, can enter the food chain, and exposure to these xenobiotics can cause a wide array of health problems in humans. Therefore, the objective of the present study was to develop an analytical method for the simultaneous determination of residual spinosad (sum of spinosyn A and D), temephos and piperonyl butoxide in porcine muscle, egg, milk, eel, flatfish and shrimp (sampling period: February to June 2018) using liquid chromatography–triple quadrupole tandem mass spectrometry (LC–MS/MS). The target analytes were extracted with a combination of acidified acetonitrile and ethyl acetate and subsequently purified with original QuEChERS kits (composed of magnesium sulfate and sodium chloride) as well as n‐hexane. All analytes were separated on a reversed‐phase analytical column using a mobile phase of (A) 0.1% formic acid containing 10 mm ammonium formate in distilled water and (B) methanol. Good linearity (R2 ≥ 0.980) was achieved over the tested concentration range (3.5–35 μg/kg for spinosyn A; 1.5–15 μg/kg for spinosyn D; 5–50 μg/kg for temephos and piperonyl butoxide) in matrix‐matched standard calibrations. Fortified samples at three spiking levels yielded recoveries in the range of 71–105% with relative standard deviations ≤9.2%. The applicability of the method was evaluated via evaluating samples collected from a large wholesale market located in Seoul, and none of the samples contained any of the target analytes. In conclusion, the current approach is simple, efficient and reliable and can successfully determine the residual levels of spinosad, temephos and piperonyl butoxide in complex animal‐derived food products. |
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Keywords: | fish LC– MS/MS milk pig muscle piperonyl butoxide residues spinosad table egg temephos |
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