基于过氧化氢-桑色素化学发光体系的硫化物检测

在pH=9～10范围内,过氧化氢与桑色素溶液混合后可产生较弱的化学发光,表面活性剂十六烷基三甲基溴化铵(CTMAB)和S2-对该体系化学发光有较强的增敏作用,据此建立了H2O2-桑色素-CTMAB化学发光体系测定S2-的新方法,并对该体系的化学发光反应机理进行了研究.在最佳的实验条件下,S2-在(0.9～9.0)×10...

Determination of Sulfide Based on Hydrogen Peroxide-morin Chemiluminescence System

In alkaline condition(pH=9~10), a weak chemiluminescence signal was produced when H₂O₂ was injected into morin solution, and the chemiluminescence signal could be greatly enhanced after the introduction of cetyl trimethyl ammonium bromide(CTMAB) and sulfide. Based on the observation, H₂O₂-morin-CTMAB chemiluminescence system has been developed for the determination of sulfide. The possible mechanism of chemiluminescence reaction has also been discussed. Under the optimized conditions, the chemiluminescence intensity of the system presented a linear relationship with the concentration of sulfide in the range of (0.9~9.0)×10^-8 g/mL, the linear dynamic equation was ΔI_CL=49.41+3.3612C_S with correlation coefficient of 0.999 0. The detection limit (S/N=3) was 1.2×10^-9 g/mL. The relative standard deviations(RSD) was 2.5％ for 8.0×10^-8 g/mL sulfide solution. The proposed method was successfully applied to the determination of sulfide in environmental water samples with recoveries in the range of 95.5%~101.3%.