用于探测生物芯片的制冷型ICCD系统

利用光锥耦合的ICCD系统探测荧光染料标记的生物芯片，并对CCD芯片和像增强器制冷，以提高探测灵敏度。基于实验分析结果，指出背景噪声的主要来源为杂散光和生物芯片基底所发的荧光，指出用镜头成像限制了系统探测灵敏度的提高，可采用低荧光物质作为生物芯片的基底对系统加以改进。

Cooled ICCD system for biochip detection

An optical cone-coupled ICCD system was developed to detect the biochips labeled with fluorescent molecules. The CCD chip and image intensifier were cooled to increase the sensitivity of the system. Based on the analyzed results of the experiment, it is determined that the major background noise comes from the stray light and the fluorescence from the substrate impurities of the biochip, and the sensitivity of the system is limited by the objective lens for imaging. The background noise can be effectively reduced and the detection sensitivity can be improved by using a substrate with less fluorescent impurities, such as a quartz substrate.