| HPLC／CoPC修饰电极安培检测大鼠心肌线粒体中谷胱甘肽的研究 |
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| 引用本文： | 施国跃 周红国. HPLC／CoPC修饰电极安培检测大鼠心肌线粒体中谷胱甘肽的研究[J]. 分析科学学报, 1999, 15(2): 94-98 |
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| 作者姓名： | 施国跃 周红国 |
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| 作者单位： | 华东师范大学化学与生命科学学院 |
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| 基金项目： | 中国科学院长春应用化学研究所电分析化学开放实验室基金 |
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| 摘 要： | 研究了钴酞菁修饰电极的修饰方法及其电化学性质。它可以催化氧化谷胱甘肽，降低ＧＳＨ的过电位。以ＣｏＰＣ何尝修饰电极为工作电极的安培薄层化学检测器，与高压认相色谱联用，在工作电极电位为＋０．８Ｖ时，ＧＳＨ的浓度在３．０＊１０＾－６ｍｏｌ／Ｌ－１．０＊１０＾－３ｍｏｌ／Ｌ浓度范围内与峰电流呈良好的线性关系，线性相关系数为０．９９９１，检出限为１．０＊１０＾－３ｍｏｌ／Ｌ浓度范围内与峰电流呈良好的线性关系
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| 关 键 词： | 谷胱甘肽 修饰电极 大鼠 心肌 线粒体 测定 |
The Study on Determination of the Glutathione in Myocardial Mitochondrion with HPLC/CoPC Modified Electrode |
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| Shi Guoyue,Zhou Hongguo,Xu Fang,Jin Litong. The Study on Determination of the Glutathione in Myocardial Mitochondrion with HPLC/CoPC Modified Electrode[J]. Journal of Analytical Science, 1999, 15(2): 94-98 |
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| Authors: | Shi Guoyue Zhou Hongguo Xu Fang Jin Litong |
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| Abstract: | In this paper, a new cobalt phthalocyanine(CoPC) electrode and its electrochemical properties are studied. It can electrocatalyze glutathione(GSH), increase the sensitivity and lower overpotential. The CoPC modified electrode is used as the working electrode of amperometric detector of HPLC, and the working potential is +0.8V(vs. Ag/AgCl). The linear correlation coefficient is 0.9991 in range of 3.010-6 mol/L to 1.010-3 mol/L with a detection limit of 1.010-6 mol/L. The application of this method in the determination of GSH in myocardial mitochondrion of rats gives an excellent result. |
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| Keywords: | CoPCmodified electrode Amperometric electrochemical detector HPLC Glutathione myocardial mitochondrion |
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