| Binding equilibrium study between Mn( Ⅱ ) and HSA or BSA |
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| 引用本文： | 梁宏,涂楚桥,张宏志,沈星灿,周永洽,申泮文. Binding equilibrium study between Mn( Ⅱ ) and HSA or BSA[J]. 中国化学, 2000, 18(1): 35-41. DOI: 10.1002/cjoc.20000180107 |
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| 作者姓名： | 梁宏 涂楚桥 张宏志 沈星灿 周永洽 申泮文 |
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| 作者单位： | Research Institute of Bioinorganic Chemistry，Department of Chemistry and Chemical Engineering，Guangxi Normal University，Guilin，Guangxi 541004，China，Research Institute of Bioinorganic Chemistry，Department of Chemistry and Chemical Engineering，Guangxi Normal University，Guilin，Guangxi 541004，China，Research Institute of Bioinorganic Chemistry，Department of Chemistry and Chemical Engineering，Guangxi Normal University，Guilin，Guangxi 541004，China，Research Institute of Bioinorganic Chemistry，Department of Chemistry and Chemical Engineering，Guangxi Normal University，Guilin，Guangxi 541004，China，Research Institute of Bioinorganic Chemistry，Department of Chemistry and Chemical Engineering，Guangxi Normal University，Guilin，Guangxi 541004，China，Department of Chemistry，Nankai University，Tianjin 300071，China |
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| 基金项目： | Project supported by the National Natural Science Foundation of China(No.29961001),the Natural Science Foundation of Guangxi Universities and the Ten,Hundred or Thousand Distinguished Persons Foundation of Guangxi. |
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| 摘 要： | The binding of Mn( Ⅱ ) to human serum albumin (HSA) or bovine serum albumin (BSA) has been studied by equilibrium dialysis at physiological pH (7. 43). The Scatchard analysis indicates that there are 1.8 and 1.9 strong binding sites of Mn( Ⅱ ) in HSA and BSA, respectively. The successive stability constants which are reported for the first time are obtained by non-linear least-squares methods fitting Bjerrum formula. For both Mn( Ⅱ )-HSA and Mn( Ⅱ )-BSA systems, the order of magnitude of K1 was found to be 104. The analyses of Hill plots and free energy coupling show that the positive cooperative effect was found in both Mn( Ⅱ )-HSA and Mn( Ⅱ )-BSA systems . The results of Mn ( Ⅱ ) competing with Cu ( Ⅱ ) 、 Zn(Ⅱ)、Cd( Ⅱ) or Ca( Ⅱ ) to bind to HSA or BSA further support the conjecture that there are two strong binding sites of Mn( Ⅱ) in both HSA and BSA. One is most probably located at the tripeptide segment of N- terminal sequence of HSA and BSA molecules involving four groups composed of n
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| 关 键 词： | Mn (Ⅱ)-serum albumin equilibrium dialysis successive stability constants positive cooperative effect coordination analysis |
Binding equilibrium study between Mn(II) and HSA or BSA |
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| LIANG,Hong TU,Chu-Qiao ZHANG,Hong-ZhiSHEN,Xing-Can ZHOU,Yong-Qia SHEN,Pan-Wen Research Institute of Bioinorganic Chemistry. Binding equilibrium study between Mn(II) and HSA or BSA[J]. Chinese Journal of Chemistry, 2000, 18(1): 35-41. DOI: 10.1002/cjoc.20000180107 |
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| Authors: | LIANG Hong TU Chu-Qiao ZHANG Hong-ZhiSHEN Xing-Can ZHOU Yong-Qia SHEN Pan-Wen Research Institute of Bioinorganic Chemistry |
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| Affiliation: | LIANG,Hong TU,Chu-Qiao ZHANG,Hong-ZhiSHEN,Xing-Can ZHOU,Yong-Qia SHEN,Pan-Wen Research Institute of Bioinorganic Chemistry,Department of Chemistry and Chemical Engineering,Guangxi Normal University,Guilin,Guangxi 541004,China Department of Chemistry,Nankai University,Tianjin 300071,China |
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| Abstract: | The binding of Mn(II) to human serum albumin (HSA) or bovine serum albumin (BSA) has been studied by equilibrium dialysis at physiological pH (7.43). The Scatchard analysis indicates that there are 1.8 and 1.9 strong binding sites of Mn(II) in HSA and BSA, respectively. The successive stability constants which are reported for the first time are obtained by non‐linear least‐squares methods fitting Bjerrum formula. For both Mn(II)‐HSA and Mn(II)‐BSA systems, the order of magnitude of K1 was found to be 104. The analyses of Hill plots and free energy coupling show that the positive cooperative effect was found in bom Mh(II)‐HSA and Mn(II)‐BSA systems. The results of Mn (II) competing with Cu (II) Zn(II), Cd(II) or Ca(II) to bind to HSA or BSA further support the conjecture that there are two strong binding sites of Mn(II) in both HSA and BSA. One is most probably located at the tripeptide segment of N‐terminal sequence of HSA and BSA molecules involving four groups composed of nitrogen atoms, and the fifth coordination atom is the carboxyl oxygen of Asp1. The coordinated atoms of the other are most probably almost all oxygen atoms. |
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| Keywords: | Mn(II)‐serum albumin equilibrium dialysis successive stability constants positive cooperative effect coordination analysis |
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