An improved ELISA for the determination of southern bean mosaic virus with linear sweep voltammetry detection based on new system of PAP-H_2O_2-HRP |
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| Authors: | ZHANG Shu-Sheng JIAO Kui CHEN Hong-YuanZHANG Cheng-Liang ZHANG Zuo-Fang |
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| Affiliation: | ZHANG,Shu-Sheng JIAO,Kui CHEN,Hong-YuanZHANG,Cheng-Liang ZHANG,Zuo-Fang Department of Chemistry,Nanjing University,Nanjing,Jiangsu 210093,China Department of Applied Chemistry,Qingdao Institute of Chemical Technology,Qingdao,Shandong 266042,China,Institute of Plant Quarantine,Ministry of Agriculture,Beijing 100029,China |
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| Abstract: | An improved enzyme‐linked immunosorbent assay (ELISA) for the determination of southern bean mosaic virus (SBMV) with linear sweep voltammetry based on a new system of p‐aminophenol (PAP)‐H2Q2‐horseradish peroxidase (HRP) has firstly been developed. The enzymatic product 3‐[(4‐hydroxyphenyl)amino]‐4‐(2‐amino‐5‐hydroxvphenyl)‐6‐[(4‐hydroxyphenyl)imino]‐2,4‐cyclohexadiene‐1‐one, produced from the oxidation of PAP with H2O2 catalyzed by HRP, yielded a sensitive linear sweep voltammetric response at ?0.45 V (vs. SCE) in Britton‐Robinson (BR) buffer solution. Based on the voltammetric peak, HRP can be measured with a detection limit of 0.4 mU/L and a linear range of 1.0–1.0×102 mU/ L. The detection limit for the SBMV is 8.0 ng/mL and the highest dilution ratio for the detection of infected leaf sap is 1: 1.5×105. |
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| Keywords: | Southern bean mosaic virus horseradish peroxidase p-aminophenol voltammetry |
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