Recent advances in fluorescence imaging of alkaline phosphatase

Phosphatase plays a vital important role in many biological functions due to the dephosphorylation serves varied roles in cellular regulation and signaling.Among the family of phosphatase,alkaline phosphatase(ALP) could act as crucial prognostic indicators for many diseases such as bone diseases and cancer.However,the detection of ALP is mainly limited to in vitro colorimetric method in clinic.Therefore,huge efforts have been paid on the fluorescence imaging that provides a reliable method to detect the real-time and in vivo changes of the level of ALP.In this review,we summarize recent advances in fluorescence imaging of phosphatase,mainly focused on ALP.The imaging probes of phosphatase are mainly classified according to their luminescence mechanisms.In the end,we assessed the challenges and future prospects of phosphatase probes.