| 液相色谱串联质谱法测定牛肉制品中猪肉含量 |
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| 引用本文： | 杜业刚,刘奕雄,李星星,孙林,王韦达,刘文丽,杨国武.液相色谱串联质谱法测定牛肉制品中猪肉含量[J].分析试验室,2021,40(1):64-69. |
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| 作者姓名： | 杜业刚 刘奕雄 李星星 孙林 王韦达 刘文丽 杨国武 |
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| 作者单位： | 深圳市计量质量检测研究院,国家营养食品质量监督检验中心(广东),深圳518100;深圳市计量质量检测研究院,国家营养食品质量监督检验中心(广东),深圳518100;深圳市计量质量检测研究院,国家营养食品质量监督检验中心(广东),深圳518100;深圳市计量质量检测研究院,国家营养食品质量监督检验中心(广东),深圳518100;深圳市计量质量检测研究院,国家营养食品质量监督检验中心(广东),深圳518100;深圳市计量质量检测研究院,国家营养食品质量监督检验中心(广东),深圳518100;深圳市计量质量检测研究院,国家营养食品质量监督检验中心(广东),深圳518100 |
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| 基金项目： | 深圳市科技计划项目(YCYJ20170817162416978);深圳市食品安全战略工程项目(深圳主食食材营养成分检测分析与大数据共享项目)资助。 |
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| 摘 要： | 建立了超高效液相色谱-串联质谱法测定牛肉制品中猪肉含量的方法。样品经蛋白提取、酶解等前处理后,在0.1%甲酸水和乙腈的梯度洗脱下经C18色谱柱分离,多反应监测模式采集质谱数据进行定量分析,线性回归系数>0.999,方法定量限低至0.5%(w:w),回收率89.6%~109%,相对标准偏差低于10%。
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| 关 键 词： | 特征肽段 液相色谱-串联质谱 牛肉 猪肉 生物标志物 |
Determination of the pork content in beef products by ultra high performance liquid chromatography-tandem mass spectrometry |
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| DU Yegang,LIU Yixiong,LI Xingxing,SUN Lin,WANG Weida,LIU Wenli,YANG Guowu.Determination of the pork content in beef products by ultra high performance liquid chromatography-tandem mass spectrometry[J].Chinese Journal of Analysis Laboratory,2021,40(1):64-69. |
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| Authors: | DU Yegang LIU Yixiong LI Xingxing SUN Lin WANG Weida LIU Wenli YANG Guowu |
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| Institution: | (Shenzhen Academy of Metrology and Quality Inspection,National Nutrition Food Testing Center(Guangdong),Shenzhen,518100) |
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| Abstract: | A method for determination of pork content in beef products by ultra high performance liquid chromatography-tandem mass spectrometry was developed.After processed by protein extraction and enzymatic hydrolysis,the samples were separated by C18 column under gradient elution with 0.1%formic acid and acetonitrile.Multiple reaction monitoring data were acquired by tandem mass spectrometry and qualitative analyzed.The correlation coefficients of linear relationship were above 0.999,limits of quantitation were as low as0.5%(w:w),the recoveries were between 89.6%and 109%,and relative standard deviations were below 10%. |
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| Keywords: | marker peptide liquid chromatography-tandem mass spectrometry beef pork biomarker |
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