| 基于多巴胺-量子点的比色免疫分析法对甲胎蛋白的高灵敏检测 |
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| 引用本文： | 杨雅妮,刘冰倩,蔡杰,罗大娟,高荣.基于多巴胺-量子点的比色免疫分析法对甲胎蛋白的高灵敏检测[J].分析试验室,2021(3):249-254. |
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| 作者姓名： | 杨雅妮 刘冰倩 蔡杰 罗大娟 高荣 |
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| 作者单位： | 贵州大学药学院 |
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| 基金项目： | 国家自然科学基金(21864007、21605029);贵州省自然科学基金(黔科合基础[2020]1042;黔科合平台人才[2017]5788、黔科合平台人才[2018]5781)项目资助。 |
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| 摘 要： | 基于抗原抗体识别特异性,以多巴胺-Mn/ZnS量子点(DA-QDs)为反应媒介,紫外可见分光光度计为检测手段,采用柠檬酸钠还原法制备金纳米颗粒(AuNPs),其表面用辣根过氧化物酶(HRP)和羊抗小鼠免疫球蛋白G (IgG)修饰获得功能化二抗(IgG-AuNPs-HRP)作为信号放大标签,构建一种简便高灵敏的检测方法....
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| 关 键 词： | 比色免疫法 多巴胺-Mn/ZnS量子点 酪胺信号放大 |
Highly sensitive detection of alpha-fetoprotein by colorimetric immunoassay based on dopamine-quantum dots |
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| YANG Yani,LIU Bingqian,CAI Jie,LUO Dajuan,GAO Rong.Highly sensitive detection of alpha-fetoprotein by colorimetric immunoassay based on dopamine-quantum dots[J].Chinese Journal of Analysis Laboratory,2021(3):249-254. |
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| Authors: | YANG Yani LIU Bingqian CAI Jie LUO Dajuan GAO Rong |
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| Institution: | (School of Pharmacy,Guizhou University,Guiyang 550025) |
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| Abstract: | Based on the specific interaction between antigen and antibody,a method for detection of alphafetoprotein was developed by using dopamine-Mn/ZnS quantum dots(DA-QDs) as reaction medium and UV-Vis spectrophotometer as detection means.Gold nanoparticles(Au NPs) were prepared by sodium citrate reduction method,and their surfaces were modified with horseradish peroxidase and goat anti-mouse immunoglobulin G to obtain a functionalized secondary antibody(IgG-AuNPs-HRP) as signal amplification label.Under optimized conditions,the response signal had a good linear relationship with the AFP concentration.The linear range was0.1-80 pg/m L,and the detection limit was 0.044 pg/m L.The recovery rates of human serum albumin ranged from 87.7% to 111.5% with the relative standard deviations of 0.2%-1.4%. |
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| Keywords: | colorimetric immunoassay dopamine-manganese/zinc sulfide quantum dots tyramine signal amplification |
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