| 多指示剂标记适体探针卡那霉素传感器的电化学行为与分析性能 |
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| 引用本文： | 崔陪,韩贤达,向阳可佳,俞志刚. 多指示剂标记适体探针卡那霉素传感器的电化学行为与分析性能[J]. 分析试验室, 2021, 40(1): 48-53 |
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| 作者姓名： | 崔陪 韩贤达 向阳可佳 俞志刚 |
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| 作者单位： | 哈尔滨理工大学化学与环境工程学院,哈尔滨150040;无机特种功能材料重庆市重点实验室博士后科研工作站,长江师范学院化学化工学院,涪陵408100;哈尔滨理工大学化学与环境工程学院,哈尔滨150040;无机特种功能材料重庆市重点实验室博士后科研工作站,长江师范学院化学化工学院,涪陵408100;哈尔滨理工大学化学与环境工程学院,哈尔滨150040;无机特种功能材料重庆市重点实验室博士后科研工作站,长江师范学院化学化工学院,涪陵408100;哈尔滨理工大学化学与环境工程学院,哈尔滨150040;无机特种功能材料重庆市重点实验室博士后科研工作站,长江师范学院化学化工学院,涪陵408100 |
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| 基金项目： | 重庆市自然科学基金(cstc2018jcyjAX0637);长江师范学院科研启动基金计划(2017KYQD122)资助。 |
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| 摘 要： | 以三分子亚甲基蓝(MB)标记适体探针为分子识别元件,构建了一种卡那霉素生物传感器.该传感器采用目标物诱导探针构型变化的信号转导机制.以Au-S化学法将适体探针自组装于金电极表面形成稳定的单分子层传感界面,利用交流伏安法和循环伏安法考察了传感过程的基础电化学行为和传感器分析性能.在优化条件下,相比于单分子MB标记,该传感...
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| 关 键 词： | 适体传感器 卡那霉素 DNA探针 电化学行为 分析性能 |
Electrochemical behavior and analytical performance of kanamycin biosensor based on multi-indicator modified aptamer probe |
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| CUI Pei,HAN Xianda,XIANG Yangkejia,YU Zhigang. Electrochemical behavior and analytical performance of kanamycin biosensor based on multi-indicator modified aptamer probe[J]. Chinese Journal of Analysis Laboratory, 2021, 40(1): 48-53 |
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| Authors: | CUI Pei HAN Xianda XIANG Yangkejia YU Zhigang |
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| Affiliation: | (College of Chemical and Environmental Engineering,Harbin University of Science and Technology,Harbin 150040;Post-Doctoral Research Center of Chongqing Key Laboratory of Inorganic Special Functional Materials,College of Chemistry and Chemical Engineering,Yangtze Normal University,Filling 408100) |
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| Abstract: | A kanamycin biosensor was fabricated using a DNA probe modified by 3 molecule methylene blue(MB)as the molecular recognition element.The sensor signaling mechanism was based on the change in probe conformation induced by the target binding.The 3 MB-modified aptamer probe was self-assembled on the Au electrode surface to form a stable sensing interface by Au-S chemistry.The basic electrochemical behavior and analytical performance of sensor system were investigated using alternate current voltammetry and cyclic voltammetry.Under the optimized conditions,the interfacial electroactivity and the current output efficiency were significantly improved compared with those obtained from MB-modified DNA probe.When used for the detection of target kanamycin,the sensor exhibited a low detection limit of 3.3 nmol/L and super-fast sensing speed with the current response equilibrium time of only 15 s,and showed high selectivity,strong anti-fouling ability,and good parallel fabrication stability.The method avoids sample pretreatment and does not need to add additional reagent. |
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| Keywords: | aptasensor kanamycin DNA probe electrochemical behavior analytical performance |
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