| VP-16与DNA分子间电子转移的光谱学研究 |
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| 引用本文： | 张蕤,史钧,汪世龙,孙晓宇,张超杰.VP-16与DNA分子间电子转移的光谱学研究[J].光谱学与光谱分析,2006,26(11):1977-1980. |
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| 作者姓名： | 张蕤 史钧 汪世龙 孙晓宇 张超杰 |
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| 作者单位： | 同济大学生命科学与技术学院,上海,200092;同济大学生命科学与技术学院,上海,200092;安徽科技学院,安徽,蚌埠,233100 |
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| 基金项目： | 国家自然科学基金
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上海市科委资助项目 |
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| 摘 要： | 运用脉冲辐解和圆二色谱技术，对用于治疗肿瘤的依托泊甙(VP-16)与鸟苷酸(GMP)和小牛胸腺DNA分子间电子转移的光谱进行了探讨。用脉冲辐解技术发现了VP-16与GMP之间的电子转移，测得两者之间的反应速率常数为3.16×107 L·mol-1·s-1,并对它们的吸收光谱进行了归属，同时用圆二色谱技术发现了VP-16与小牛胸腺DNA之间相互作用。从而为医学工作者进一步了解和探讨VP-16的抗肿瘤机理提供了直接的理论依据。
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| 关 键 词： | 鬼臼毒素 VP-16 脉冲辐解 抗肿瘤活性 圆二色谱 |
| 文章编号： | 1000-0593（2006）11-1977-04 |
| 收稿时间： | 2005-09-06 |
| 修稿时间： | 2005-12-09 |
Studies on the Electron Transfer between Etoposide (VP-16) and DNA |
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| ZHANG Rui,SHI Jun,WANG Shi-long,SUN Xiao-yu,ZHANG Chao-jie.Studies on the Electron Transfer between Etoposide (VP-16) and DNA[J].Spectroscopy and Spectral Analysis,2006,26(11):1977-1980. |
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| Authors: | ZHANG Rui SHI Jun WANG Shi-long SUN Xiao-yu ZHANG Chao-jie |
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| Institution: | 1. School of Life Science and Technology, Tongji University, Shanghai 200092, China2. Anhui Sicence and Technology University, Bengbu 233100, China |
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| Abstract: | In the present study, the electron transfer between Etoposide (VP-16) and GMP or DNA was investigated using pulse radiolysis and circular dichroism technology. The electron transfer between VP-16 and GMP was found, and the reaction rate constant was determined as 3.16 x 10(7) L x mol(-1) x s(-1) by pulse radiolysis. The authors found the interaction of VP-16 and DNA using the technology of circular dichroism. This study has provided theoretical reference for further study on the anti-tumor mechanism of VP-16. |
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| Keywords: | Podophyllotoxin Etoposide(VP-16) Pulse radiolysis Anti-tumor activity Circular dichroism |
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