Development of a Temperature Shift Strategy for Efficient Docosahexaenoic Acid Production by a Marine Fungoid Protist, <Emphasis Type="Italic">Schizochytrium</Emphasis> sp. HX-308 |
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Authors: | Yan Zeng Xiao-Jun Ji Min Lian Lu-Jing Ren Li-Jing Jin Ping-Kai Ouyang He Huang |
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Institution: | (1) State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, No. 5 Xinmofan Road, Nanjing, 210009, People’s Republic of China; |
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Abstract: | Temperature was one of the important environmental factors affecting the biosynthesis of docosahexaenoic acid (DHA; C22:6,
ω−3). Generally, a low temperature would slow the strain growth, but promote the accumulation of unsaturated fatty acids.
According to this information, the effects of temperature and different two-stage temperature shifting strategies on fatty
acid production and DHA content of the marine fungoid protist, Schizochytrium sp. HX-308, were investigated in this study. Finally, the highest DHA percentage was up to 51.98% (per total fatty acids)
with the DHA production of 6.05% (per dry cell weight), which was obtained with the method of shifting temperature from 30 °C
for 32 h to 20 °C for 12 h. |
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