Triggering of the Antibacterial Activity of Bacillus subtilis B38 Strain against Methicillin-Resistant Staphylococcus aureus |
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Authors: | Olfa Tabbene Ines Karkouch Imen Ben Slimene Najib Elfeddy Pascal Cosette Maria-Luisa Mangoni Thierry Jouenne Ferid Limam |
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Affiliation: | 1.Laboratoire des Substances Bioactives,Centre de Biotechnologie de Borj-Cedria,Hammam-lif Cedex,Tunisia;2.Unité de Recherche Agro-Biotechnologie,Laboratoire Phytobactériologie, INRA,Marrakech,Morocco;3.UMR 6270 CNRS, Faculté des Sciences,Université de Rouen,Mont Saint Aignan Cedex,France;4.Dipartimento di Scienze Biochimiche,Università La Sapienza,Rome,Italy |
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Abstract: | When cultured in minimal growth medium, the B38 strain of Bacillus subtilis did not exhibit any antibacterial activity against methicillin-resistant Staphylococcus aureus (MRSA) clinical isolate. Coculturing B38 strain with viable MRSA cells weakly increased antibacterial activity production (20 AU/ml). Addition of dead MRSA cells in a B38 culture, increased by 8-fold the B. subtilis strain antibacterial activity reaching 160 AU/ml against MRSA strain. This antibacterial activity recovered from cell-free supernatants was stimulated by an autoinducing compound which is sensitive to the action of proteinase K suggesting a proteinaceous nature. This compound was heat-stable till 80 °C and showed a molecular mass around 20 kDa as determined by SDS-PAGE. These results suggest that the production of antibacterial compounds by B38 strain is dependent on the amount of the autoinducing compound. |
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