| 纳米TiO2光催化氧化对胃癌细胞周期的影响 |
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| 引用本文： | 文雯,高晓亚,张爱平,宋志英,孙彦平. 纳米TiO2光催化氧化对胃癌细胞周期的影响[J]. 应用化学, 2012, 29(6): 663-667. DOI: 10.3724/SP.J.1095.2012.00324 |
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| 作者姓名： | 文雯 高晓亚 张爱平 宋志英 孙彦平 |
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| 作者单位： | (1.山西医科大学药学院 太原 030001；2.太太原理工大学洁净化工研究所 太原 030024) |
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| 基金项目： | 山西省自然科学基金(2010011048-1);山西医科大学科技创新基金(01200806)资助项目 |
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| 摘 要： | 以胃癌细胞系BGC-823为研究对象,采用倒置相差显微镜观察纳米TiO2光催化氧化前后BGC-823细胞形态的变化,通过流式细胞仪考察其对BGC-823细胞凋亡率及细胞周期的影响。结果表明,纳米TiO2光催化氧化使BGC-823细胞呈受激坏死或凋亡晚期继发性坏死状态;导致BGC-823细胞周期阻滞于G2/M期并诱导细胞凋亡,从而抑制其DNA合成及增殖分化进程。当TiO2比负载量为9.8×10-7g/cm2、光照时间为40 min时,G2/M期阻滞较为显著,细胞凋亡率达24.34%。
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| 关 键 词： | 光催化氧化 纳米TiO2 胃癌细胞BGC-823 细胞周期 细胞凋亡 |
| 收稿时间： | 2011-07-22 |
Effects of Nano-TiO2 Photocatalytic Oxidization on the Cell Cycle of BGC-823 Gastric Cancer Cells |
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| WEN Wen , GAO Xiaoya , ZHANG Aiping , SONG Zhiying , SUN Yanping. Effects of Nano-TiO2 Photocatalytic Oxidization on the Cell Cycle of BGC-823 Gastric Cancer Cells[J]. Chinese Journal of Applied Chemistry, 2012, 29(6): 663-667. DOI: 10.3724/SP.J.1095.2012.00324 |
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| Authors: | WEN Wen GAO Xiaoya ZHANG Aiping SONG Zhiying SUN Yanping |
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| Affiliation: | (1.School of Pharmacy,Shanxi Medical University,Taiyuan 030001,China;2.Institute of Clean Technique for Chemical Engineering of Taiyuan University of Technology,Taiyuan 030024,China) |
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| Abstract: | In order to explore the mechanisms of killing cancer cells via nano-TiO2 photocatalytic oxidization,gastric cancer cell line BGC-823 was cultured as research object in this study.The morphologic changes were observed by inverted phase-contrast microscope,the apoptotic rates and cell cycle of BGC-823 cells were examined by flow cytometer.The results showed that nano-TiO2 photocatalytic oxidation caused the necrosis or secondary necrosis of BGC-823 cells,and inhibited the synthesis of DNA and their proliferation process,through arresting the G2/M phase and inducing apoptosis of BGC-823 cells.Significant arrest was observed and the apoptotic rates of BGC-823 cells reached 24.34% when the coated amount of TiO2 was 9.8×10-7 g/cm2 and the irradiation time was 40 min. |
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| Keywords: | photocatalytic oxidization nano-TiO2 gastric cancer cell line BGC-823 cell cycle apoptosis |
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