DNA-modified electrodes. Part 6. A new method for the determination of binding constants and binding site sizes

A new method was developed to obtain the binding constant (K) and binding site size (n) for the interaction of DNA with other molecules using DNA-modified gold electrodes. This method is simple and microsample-consuming compared with conventional solution methods. A Q-basic program was designed to calculate K and n values. The results show that the K and n values obtained by the new method approach those obtained by conventional electrochemical methods, indicating the suitability of this method. Received: 22 July 1998 / Revised: 24 November 1998 / Accepted: 27 November 1998