固相微萃取-气相色谱-质谱法鉴别海南黄花梨木与越南黄花梨木

采用固相微萃取-气相色谱-质谱法(SPME-GC-MS)研究了海南黄花梨木与越南黄花梨木中的挥发性成分,并对二者进行了鉴别。用电钻在黄花梨木上钻取少量粉末,采用SPME富集挥发性成分,经AB-FFAP极性毛细管气相色谱柱分离,共鉴定出20种挥发性成分,并发现了可用于鉴别海南黄花梨木与越南黄花梨木的两对特征成分:2-(1,1-二甲基乙基)-5-庚基-5-甲基-1,3-二氧戊环-4-酮与2,6,10-三甲基-7,10-环氧-2,11-十二碳二烯-6-醇、2-甲基亚丁基-环戊烷与2-(5-甲基-2-呋喃基)丙醛,并利用其相对含量的关系对海南黄花梨木与越南黄花梨木进行鉴别。该方法具有样品用量小、操作简便快速、不损害整体结构等特点,可用于黄花梨木挥发性成分的分析研究,亦适用于海南黄花梨木与越南黄花梨木的树种鉴别,在家具、工艺品及原木等的鉴别中具有广泛的应用前景。

Identification of dalbergia odorifera and dalbergia spp by solid phase microextraction-gas chromatography-mass spectrometry

In addition to species identification, the volatile components of dalbergia odorifera and dalbergia spp, were investigated by solid phase microextraction-gas chromatography-mass spectrometry (SPME-GC-MS). Small amounts of powder samples were acquired by drilling from dalbergia, then the volatile components of the powder samples were concentrated by SPME, and separated by AB-FFAP polar capillary GC column. Of all the volatile components, twenty types were identified, and two pairs of them, 2-(1,1-dimethylethyl)-5-heptyl-5-methyl-1,3-dioxolan-4-one and 2,6,10-trimethyl-7,10-epoxy-2,11-dodecadien-6-ol, 2-methylbutylidene-cyclopentane and 2-(5-methyl-furan-2-yl)-propionaldehyde, could be considered specific volatile components for the identification of dalbergia odorifera and dalbergia spp, and the relative contents of these two pairs were compared for the species identification analysis. The method has the advantages of less sample requirement, simple operation, and no construction damage. The method is suitable for the research of volatile components and species identification analysis of dalbergia odorifera and dalbergia spp, and has broad prospective application in the analysis of furniture, art collections and raw wood of dalbergia.