亲水相互作用色谱-串联质谱法测定液体食品中14种甜味剂

建立了亲水相互作用色谱-三重四极杆质谱（HILIC-MS/MS）同时测定液体食品中6种人工合成甜味剂和8种甜菊糖苷类天然甜味剂的分析方法。样品经Waters Xbridge Amide色谱柱（150 mm×5.0 mm，3.5 μm）分离，以乙腈-10 mmol/L甲酸铵溶液（65：35，v/v）为流动相，流速为0.4 mL/min，柱温为35℃，然后以电喷雾电离（ESI）源，在多反应监测（MRM）、负离子模式下进行三重四极杆质谱检测。14种甜味剂在各自的范围内线性关系良好，相关系数均大于0.995，检出限为0.03~0.7 mg/kg，定量限为0.1~2.2 mg/kg。在2、5和20 mg/kg添加水平下，14种甜味剂的平均回收率为80.8%~108.7%，相对标准偏差为1.5%~7.7%（n=6）。该方法样品前处理操作简单，准确度高，灵敏度高，可用于液体食品中14种甜味剂的同时测定。

Determination of 14 sweeteners in liquid foods by hydrophilic interaction liquid chromatography-tandem mass spectrometry

A method for the simultaneous determination of six artificial sweeteners and eight steviol glycosides in liquid food samples by hydrophilic interaction liquid chromatography-tandem mass spectrometry (HILIC-MS/MS) was developed.The sweeteners in the samples were separated by a Waters Xbridge Amide column (150 mm×5.0 mm, 3.5 μm) at 35℃, and eluted with acetonitrile-10 mmol/L ammonium formate (65:35, v/v) at a flow rate of 0.4 mL/min.Then, the analytes were detected using an electrospray ion source in negative mode (ESI^-) under multiple reaction monitoring (MRM) mode.Good linear relationships were obtained, and the correlation coefficients (r²) of the 14 sweeteners were greater than 0.995.The limits of detection (LODs, S/N=3) and the limits of quantification (LOQs, S/N=10) ranged from 0.03 to 0.7 mg/kg and 0.1 to 2.2 mg/kg, respectively.The average recoveries varied from 80.8% to 108.7% at spiked levels of 2, 5 and 20 mg/kg, and the relative standard deviations (RSDs) ranged from 1.5% to 7.7%(n=6).The proposed method is simple, accurate and sensitive for the simultaneous determination of the 14 sweeteners in liquid food samples.