Interaction of Fluorescein with Felodipine: A Spectrofluorometric and Thermodynamic Study |
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| Authors: | U S Mote S L Bhattar S R Patil G B Kolekar |
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| Institution: | (1) Fluorescence Spectroscopy Research Laboratory, Department of Chemistry, Shivaji University, Kolhapur, 416004, MS, India |
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| Abstract: | The interaction between fluorescein and felodipine (FLD) was investigated by the spectrofluorometric method. The fluorescence
of FLD was quenched by fluorescein and quenching is in accordance with the Stern-Volmer relation. The binding constants of
fluorescein with FLD were obtained at different temperatures. The binding constant and number of binding sites at different
temperatures were calculated yielding the corresponding thermodynamic parameters ΔS, ΔH and ΔG. The distance r between the donor (FLD) and acceptor (fluorescein) molecules was obtained according to the fluorescence resonance energy
transfer. The optimum conditions for the fluorometric determination of fluorescein were studied and the quenching method was
successfully applied to estimate the fluorescein concentration of the pharmaceutical sample directly. |
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| Keywords: | Thermodynamic parameter Felodipine Fluorescein Binding constant Fluorescence quenching Stern-Volmer constant |
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