| 电化学发光淬灭法测定SOD |
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| 引用本文： | 储海虹,吴莹,狄俊伟,屠一锋.电化学发光淬灭法测定SOD[J].分析测试学报,2006,25(1):125-126,129. |
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| 作者姓名： | 储海虹 吴莹 狄俊伟 屠一锋 |
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| 作者单位： | 苏州大学,化学化工学院,江苏,苏州,215006 |
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| 摘 要： | 在硼酸缓冲溶液（pH6．7）中性介质中，O2和H2O2均对鲁米诺的电化学发光（ECL）有敏化作用，而超氧化物歧化酶（SOD）对此敏化的电化学发光具有淬灭作用，SOD的浓度与两种发光体系的ECL光强成线性关系，可用于SOD的测定，为SOD的测定提供了一种灵敏、可靠的测定方法。
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| 关 键 词： | 鲁米诺 电化学发光 超氧化物歧化酶 淬灭 |
| 文章编号： | 1004-4957（2006）01-0125-02 |
| 收稿时间： | 2005-01-19 |
| 修稿时间： | 2005-01-192005-09-30 |
Determination of SOD by Electrochemiluminescence Quenching Analysis |
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| CHU Hai-hong,WU Ying,DI Jun-wei,TU Yi-feng.Determination of SOD by Electrochemiluminescence Quenching Analysis[J].Journal of Instrumental Analysis,2006,25(1):125-126,129. |
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| Authors: | CHU Hai-hong WU Ying DI Jun-wei TU Yi-feng |
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| Institution: | College of Chemistry and Chemical Engineering, Suzhou University, Suzhou 215006, China |
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| Abstract: | It was found that the sensitized luminol electrochemiluminescence(ECL) formed through the reaction of dissolved oxygen or hydrogen peroxide with luminol at a boric acid buffer solution of pH 6.7,could be quenched by SOD.A sensitive and reliable method based on the above phenomenon was thus established for the determination of SOD.The sensitized ECL intensity has a linear relationship with the SOD concentration in the range of 0.24-9.6 IU/mL for luminol-O_2 system,and in the range of 0.024-1.8 IU/mL for luminol-H_2O_2 system.The mechanism of the quenching effect of SOD on the sensitized luminol ECL was also studied. |
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| Keywords: | Luminol Electrochemiluminescence SOD Quenching |
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