Separation of racemic 2,4-dinitrophenyl amino acids on zirconia-immobilized quinine carbamate in reversed-phase liquid chromatography |
| |
Authors: | Park Jung Hag Lee Joon Woo Song Young Tae Ra Chun Sup Cha Jin Soon Ryoo Jae Jeong Lee Wonjae Kim In Whan Jang Myung Duk |
| |
Affiliation: | Yeungnam University, Department of Chemistry, Kyongsan 712-749, Korea. jhpark@yu.ac.kr |
| |
Abstract: | Zirconia is known to be one of the best chromatographic support materials due to its excellent chemical, thermal, and mechanical stability. A quinine carbamate-coated zirconia was prepared as a chiral stationary phase for separation of enantiomers of DNP-amino acids in reversed-phase liquid chromatography. Retention and enantioselectivity of this phase were compared to those for quinine carbamate bonded onto silica. Most amino acids studied were separated on the quinine carbamate-zirconia CSP although retention was longer and chiral selectivity was somewhat lower than on the corresponding silica CSP. Increased retention and decreased selectivity are probably due to strong non-enantioselective Lewis acid-base interactions between the amino acid molecule and the residual Lewis acid sites on the zirconia surface. |
| |
Keywords: | Quinine carbamate Zirconia Chiral stationary phase DNP‐amino acids Reversed‐phase liquid chromatography |
本文献已被 PubMed 等数据库收录! |