Highly sensitive detection of microRNA by chemiluminescence based on enzymatic polymerization |
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| Authors: | Ma Changbei Yeung Edward S Qi Shengda Han Rui |
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| Institution: | (1) Ames Laboratory-USDOE and Department of Chemistry, Iowa State University, Ames, IA 50011, USA; |
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| Abstract: | We have developed a new methodology for miRNA assay using chemiluminescence imaging by poly(U) polymerase catalyzed miRNA
polymerization. This method is very sensitive with a 50 fM limit of detection, which is comparable to or better than current
assay methods. Multiplex detection for miRNA can be easily realized by introducing different capture probes onto the biosensor
array, which will make it highly versatile for various research purposes. |
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