A novel method for fast determination of vitamin B6 by fast continuous cyclic voltammetry |
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Authors: | M R Ganjali P Norouzi S Shirvani-Arani A Mohammadi |
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Institution: | (1) Center of Excellence in Electrochemistry, Department of Chemistry, University of Tehran, Tehran, Iran |
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Abstract: | In this work a novel method for the determination of Vitamin B6 in flow-injection systems has been developed. The fast Fourier
transform continuous cyclic voltammetry (FFTCV) at gold microelectrode in flowing solution system was used for determination
of Vitamin B6. This method is rapid, simple and highly sensitive procedures allowing the determination of Vitamin B6 in pharmaceutical
analysis. The effects of various parameters on the sensitivity of the method were investigated. The best performance was obtained
with the pH value of 2, scan rate value of 30 V/s, accumulation potential of 200 mV and accumulation time of 0.3 s. The proposed
method has some advantages over other reported methods such as, no need for the removal of oxygen from the test solution,
a sub-nanomolar detection limit, and finally the method is fast enough for the determination of any such compound, in a wide
variety of chromatographic methods. To obtain a sensitive determination, the integration range of currents was set for all
the potential scan ranges, including oxidation and reduction of the Au surface electrode, while performing the measurements.
The potential waveform, consisting of the potential steps for cleaning, accumulation and potential ramp of analyte, was applied
on an Au disk microelectrode (12.5 μm in radius) in a continuous way. The detection limit of the method for Vitamin B6 was
2.8 pg/ml. The relative standard deviation of the method at 2.1% was 8 runs.
Published in Russian in Elektrokhimiya, 2008, Vol. 44, No. 2, pp. 173–181.
The text was submitted by the authors in English. |
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Keywords: | vitamin B6 continuous cyclic voltammetry gold electrode |
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