Determination of mercury(II) traces in drinking water by inhibition of an urease reactor in a flow injection analysis (FIA) system |
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Authors: | Renbing Shi K Stein Georg Schwedt |
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Institution: | (1) Institute of Inorganic and Analytical Chemistry, TU Clausthal, Paul-Ernst-Strasse 4, D-38678 Clausthal-Zellerfeld, Germany, DE;(2) Department of Non-Timber Forest, Central-South Forestry University, 412006 Zhuzhau, Hunan, People’s Republic of China, CN |
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Abstract: | The integration of an urease reactor into a gas diffusion flow injection system was investigated for the determination of
urease inhibitors. The enzyme was immobilized by entrapping in polyacrylamide gel. Besides copper and silver ions mercury
ions inhibit the conversion of urea to carbon dioxide and ammonia catalysed by urease. The pH change of the carrier solution
caused by the ammonia released was measured potentiometrically with a pH electrode. The inhibition behaviour of Hg(II) ions
was investigated. A linear range from 2 to 20 μg L-1 Hg(II) was obtained after a 90 s inhibition, with a correlation coefficient of r=0.9997. The relative standard deviation
was 1.4% for five measurements of 2 μg L-1Hg(II). A sample frequency of 7 h-1 was achieved. The inhibited enzyme can be reactivated. The method was applied to the determination of Hg(II) in two drinking
water samples.
Received: 16 April 1996/Revised: 3 June 1996/Accepted: 11 June 1996 |
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