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Gel-Electrophoresis and Subsequent Optical Emission 15N Analysis to Identify 15N-Labelled Protein Fractions |
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| Authors: | Á Bálint Gy Heltai K Jung E Nótás |
| Institution: | 1. Department of Chemistry and Biochemistry , University of Agricultural Sciences , G?d?ll?, Hungary;2. Department of Chemical Ecotoxicology , Centre for Environmental Research , Leipzig-Halle Ltd., Leipzig, Germany |
| Abstract: | Abstract A combined procedure to detect of 15N/14N isotope ratios by emission spectrometric analysis after starch gel-electrophoresis was developed. 15N-labelled proteins of human serum were used to optimise this method. Electrophorised gel slices with protein fractions were directly digested for subsequent isotope analysis. This method is proposed for use in routine analysis for clinical application. |
| Keywords: | Analysis emission spectrometry gel-electrophoresis nitrogen 15 proteins |