Helicobacter Pylori in Vivo Diagnosis after Food Intake: Results of Simultaneous [15N]Urea Urine and [13C]Acetate Breath Tests |
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| Authors: | P Krumbiegel D M Müller |
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| Institution: | 1. Department of Human Exposure Research and Epidemiology , UFZ Centre for Environmental Research , Leipzig-Halle, Leipzig, Germany;2. Department of Paediatrics , University of Leipzig , Leipzig, Germany |
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| Abstract: | Abstract The protocols for 13C and 15N H. pylori tests stipulate that the diagnostic agent should be taken on an empty stomach. It is presumed that food intake prior to the tests leads to less reliable test results due to a prolongation of the gastric residence time of the diagnostic agent urea. This might allow the bacteria to split a higher proportion of urea, resulting in an increased number of false positives. 12 probands received 150mg 15N]urea and 75 mg sodium 13C]acetate in 75 ml orange juice as a test drink. 15N]Urea served as an agent to diagnose gastric H. pylori colonization. The 15N tests were evaluated using a urine sample of the second hour after test start. 13C]Acetate served as a marker of the gastric emptying of water-soluble food including the urea under the influence of food intake. Breath air samples were taken to calculate the gastric emptying half life (EHL) and the apparent resorption time (RT) of the urea. The double tests were carried out four times within four weeks using identical test protocols but different |
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| Keywords: | Body water Hydrogen-2 Isotope dilution Men |
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