Estimation of N Absorption and Secretion by Digesta Exchange between 15N Labelled and Unlabelled Pigs |
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| Authors: | K Krawielitzki T Zebrowska R Schadereit J Kowalczyk F Kreienbring |
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| Institution: | 1. Forschungsinstitut für Biologie landwirtschaltlicher Nutztiere Dummerstorf-Rostock, FB Ern?hrungsphysiologie , ?Oskar Kellner“, Rostock, Germany;2. Promoted in Scientists Integration Programme (WIP) “Protein Turnover” by KAI e.V. , Berlin, Germany;3. Kielanowski Institute of Animal Physiology and Nutrition Jablonna/Warsaw Polish Academy of Science , Poland;4. Forschungsinstitut für Biologie landwirtschaltlicher Nutztiere Dummerstorf-Rostock, FB Ern?hrungsphysiologie , ?Oskar Kellner“, Rostock, Germany |
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| Abstract: | Abstract Three female pigs (LW～30 kg) were fitted with re-entrant cannulas in the duodenum and the ileum and with bladder catheters. Animal No. 1 was labelled by continuous infusion of 15N]leucine via a catheter into the vena jugularis. After reaching a steady state in the level of endogenous N (4–5 d after beginning of the infusion) the digesta of the labelled animal No. 1 and the two unlabelled animals were exchanged in a 3 day experiment. During this time the course of transit rates of digesta, digesta N and 15N through duodenum and ileum as well as the proportion of endogenous N: exogenous N were estimated. Using these data it was possible to calculate the secretion and absorption rates of endogenous and exogenous N in the three segments of the digestive tract: mouth-duodenum, duodenum-ileum, ileum-anus and in addition the reabsorption (intestinal recycling) of the endogenous N during its passage through the gut could be computed. |
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| Keywords: | Absorption digesta endogenous N exogenous N intestine nitrogen turnover nitrogen 15 pigs tracer techniques transit rate |
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