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Determination of 13C Enrichment by Conventional GC-MS and GC-(MS)-C-IRMS |
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| Authors: | W Meier-augenstein D Rating G F Hoffmann U Wendel U Matthiesen P Schadewaldt |
| Institution: | 1. Dept. of Anatomy &2. Physiology, University of Dundee , Great Britain;3. Kinderklinik der Ruprecht-Karls-Universit?t Heidelberg , Deutschland;4. Kindergeneeskunde, Akademisch Ziekenhuis, Universiteit Nijmegen , The Netherlands;5. Spurenelementlabor, Heinrich-Heine-Universit?t Düsseldorf , Deutschland;6. Diabetesforschungsinstitut, Heinrich-Heine-Universit?t Düsseldorf , Deutschland |
| Abstract: | Abstract Isotopic enrichment of branched-chain L-amino acids (BCAA) and branched-chain 2-oxo acids (BCOA) in standard preparations and in human plasma samples withdrawn after oral loads with 1-13C labelled BCAA were measured on a conventional GC-MS system and an on-line GC-C-IRMS employing O-TMS quinoxalinol derivatives. It was concluded that the recently introduced GC-C-IRMS, owing to its high sensitivity, is the adequate analytical tool when tracer doses of stable isotope labelled compounds of low enrichment are to be used in biomedical in vivo studies. |
| Keywords: | branched-chain amino acids GC-C-IRMS GC-MS MSUD stable isotope analysis selected ion monitoring tracer techniques |