Effects of N-application on utilization of 15N and 13C and quality in two wheat cultivars |
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| Authors: | Klaus-Peter Götz Helmut Herzog Osman Erekul |
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| Institution: | 1. Department of Crop Science in the Tropics and Subtropics , Humboldt Universit?t zu Berlin , Albrecht-Thaer-Weg 5, 14795, Berlin, Germany klaus-peter.goetz@agrar.hu-berlin.de;3. Department of Crop Science in the Tropics and Subtropics , Humboldt Universit?t zu Berlin , Albrecht-Thaer-Weg 5, 14795, Berlin, Germany;4. Department of Crop Science , Faculty of Agriculture, Adnan Menderes University , 09100, Aydin, Turkey |
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| Abstract: | We studied N uptake and distribution in wheat, and the incorporation of nitrogen and carbon into gluten and non-gluten proteins using a double-labelling approach with 15N and 13C. Doses of N-fertilizer were split and applied at emergence, onset of stem elongation, and heading at rates of 280/140/140 mg N pot?1, respectively simulating 90/45/45 kg N ha?1. Five different combinations of N-fertilizations containing no or 10 % 15N were performed. The recovery of 15N added at the stages emergence, stem elongation or heading were 42, 60, and 64 %. Application of 15N at all three stages yielded in 51 % recovery. Remobilisation of straw N was greater for Golia. The 15N concentration in gluten proteins of Golia show higher values than Gönen. The ratio of 15N gluten/15N non-gluten proteins of Golia were higher, which implies a lower non-gluten protein activity during grain filling. The 13C concentration in gluten and non-gluten proteins did not differ between both cultivars. |
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| Keywords: | carbon-13 gluten-proteins nitrogen-15 N-fertilizer N-uptake N-utilization non-gluten proteins wheat |
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