Simulating the sensitivity of cell nutritive environment to composition changes within the intervertebral disc |
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| Affiliation: | 1. Department of Communication and information Technologies (DTIC), Universitat Pompeu Fabra (UPF), Barcelona, Spain;2. Institute for Bioengineering of Catalonia (IBEC), Barcelona, Spain;3. Department of Mechanical Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA;4. Department of Biomedical Engineering, Eindhoven University of Technology, Eindhoven, The Netherlands;5. INSIGNEO Institute for In Silico Medicine, Department of Mechanical Engineering, University of Sheffield, Sheffield, UK;1. Institute for Surgical Technology and Biomechanics, University of Bern, Switzerland;2. Department of Clinical Research, University of Bern, Switzerland;3. Institute of Forensic Medicine, University of Zurich, Switzerland;1. Department of Mechanical and Aerospace Engineering, University of Miami, Coral Gables, FL, USA;2. Department of Biomedical Engineering, University of Miami, Coral Gables, FL, USA;1. Institute for Biomechanics, ETH Zurich, Hönggerbergring 64, HPP-O14, 8093 Zurich, Switzerland;2. Institute of Biomechanics, TUHH Hamburg University of Technology, Denickestraße 15, K, 21073 Hamburg, Germany;3. Institute for Forensic Medicine, University Medical Center Hamburg—Eppendorf, Butenfeld 34, D-22529 Hamburg, Germany |
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| Abstract: | Altered nutrition in the intervertebral disc affects cell viability and can generate catabolic cascades contributing to extracellular matrix (ECM) degradation. Such degradation is expected to affect couplings between disc mechanics and nutrition, contributing to accelerate degenerative processes. However, the relation of ECM changes to major biophysical events within the loaded disc remains unclear. A L4-L5 disc finite element model including the nucleus (NP), annulus (AF) and endplates was used and coupled to a transport-cell viability model. Solute concentrations and cell viability were evaluated along the mid-sagittal plane path. A design of experiment (DOE) was performed. DOE parameters corresponded to AF and NP biochemical tissue measurements in discs with different degeneration grades. Cell viability was not affected by any parameter combinations defined. Nonetheless, the initial water content was the parameter that affected the most the solute contents, especially glucose. Calculations showed that altered NP composition could negatively affect AF cell nutrition. Results suggested that AF and NP tissue degeneration are not critical to nutrition-related cell viability at early-stage of disc degeneration. However, small ECM degenerative changes may alter significantly disc nutrition under mechanical loads. Coupling disc mechano-transport simulations and enzyme expression studies could allow identifying spatiotemporal sequences related to tissue catabolism. |
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| Keywords: | Intervertebral disc degeneration Tissue composition Finite element analysis Cell nutrition Multiphysics |
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