Potentiometric immunosensor based on antiserum of Japanese B encephalitis immobilized in nano-Au/polymerized o-phenylenediamine film |
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| Authors: | Lingyan Zhang Ruo Yuan Xiaoqing Huang Yaqing Chai Shurui Cao |
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| Institution: | aChongqing Key Laboratory of Analytical Chemistry, College of Chemistry and Chemical Engineering, Southwest China Normal University, Chongqing 400715, China;bSchool of Material Science and Chemical Engineering, Tianjin Polytechnic University, Tianjin 300160, China |
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| Abstract: | A novel potentiometric immunosensor for detection of Japanese B encephalitis vaccine was developed by immobilizing antiserum of Japanese B encephalitis on nano-Au/polymerized o-phenylenediamine (o-PDA) film on the platinum (Pt) electrode. The performance and factors influencing the performance of the resulting immunosensor were studied. The immunosensor showed a specific response to Japanese B encephalitis vaccine in the range 1.1 × 10−8 to 2.0 × 10−6 lgpfu/ml (plaque forming unit) with a detection limit of 6 × 10−9 lgpfu/ml. The correlation coefficient is 0.9986. The incubation time, incubation temperature, pH, reproducibility and stability of the immunosensor were also studied. The present work supplied a promising test method for biological products. |
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| Keywords: | Potentiometric Nano-Au Japanese B encephalitis vaccine Immunosensor |
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