CSE-MECC two-dimensional capillary electrophoresis analysis of proteins in the mouse tumor cell (AtT-20) homogenate |
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Authors: | Chen Xingguo Fazal Md Abul Dovichi Norman J |
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Affiliation: | aDepartment of Chemistry, University of Washington, Seattle, WA 98195, USA |
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Abstract: | Two-dimensional capillary electrophoresis was used for the separation of proteins and biogenic amines from the mouse AtT-20 cell line. The first-dimension capillary contained a TRIS–CHES–SDS–dextran buffer to perform capillary sieving electrophoresis, which is based on molecular weight of proteins. The second-dimension capillary contained a TRIS–CHES–SDS buffer for micellar electrokinetic capillary chromatography. After a 61-s preliminary separation, fractions from the first-dimension capillary were successively transferred to the second-dimension capillary, where they further separated by MECC. The two-dimensional separation required 60 min. |
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Keywords: | Capillary electrophoresis Two-dimensional electrophoresis AtT-20 cell line |
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