Intramolecular dimers: a new design strategy for fluorescence-quenched probes |
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Authors: | Johansson Mary Katherine Cook Ronald M |
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Affiliation: | Biosearch Technologies, 81 Digital Drive, Novato, CA 94949, USA. info@biosearchtech.com |
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Abstract: | Fluorogenic probes dual-labeled with reporter and quencher dyes use a change in fluorescence to monitor biochemical events (e.g., substrate binding or enzyme digestion). Such events change the reporter-quencher distance, which affects fluorescence. Recently, it is has been shown that static quenching through intramolecular dimers is an important mechanism that can sometimes be more efficient than F?rster resonance energy transfer (FRET). |
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Keywords: | biosensors fluorescent probes FRET (fluoresence resonance energy transfer) intramolecular dimer stacking interactions |
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