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Optimization of the capillary gas chromatographic analysis of mono- and oligosaccharides in honeys
Authors:J A Gómez Bárez  R J Garcia-Villanova  S Elvira Garcia  A M González Paramás
Institution:(1) Departamento de Quimíca Analítica, Nutrición y Bromatología, Facultad de Farmacia, Universidad de Salamanca, Campus M. de Unamuno, 37007 Salamanca, Spain
Abstract:Summary A capillary gas chromatographic method has been developed for simultaneous determination of at least 18 sugars (2 monosaccharides, 11 disaccharides and 5 trisaccharides) in honey, as their oxime-trimethylsilyl ethers. The chromatographic conditions have been systematically and thoroughly optimized, to eliminate the peak overlap commonly reported for this and other methods, especially for disaccharides. Complete analytical details are reported.
Keywords:Gas chromatography  Honey sugars  Oxime-trimethylsilyl ethers of sugars  Mono-  di- and trisaccharides
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