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A one-step immunochromatographic assay for detecting ginsenosides Rb1 and Rg1 |
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| Authors: | Waraporn?Putalun Noriko?Fukuda Hiroyuki?Tanaka |
| Institution: | (1) Faculty of Pharmaceutical Sciences, Khon Kaen University, 40002 Khon Kaen, Thailand;(2) Graduate School of Pharmaceutical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, 812-8582 Fukuoka, Japan |
| Abstract: | An immunochromatographic strip test has been developed for detecting ginsenosides Rb1 (G-Rb1) and Rg1 (G-Rg1). This qualitative assay system is useful as a rapid screening method for detecting G-Rb1 and G-Rg1 in plants and plant preparations. Our assay is a competitive immunoassay that uses anti-G-Rb1 and anti-G-Rg1 monoclonal antibodies (MAbs) and a detection reagent that contains colloidal gold particles coated with anti-G-Rb1 and anti-G-Rg1 MAbs. Detection limits are 2  g mL–1 for both G-Rb1 and G-Rg1. |
| Keywords: | Ginsenoside Rb1 Ginsenoside Rg1 Immunochromatographic |
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