|
|||||
|
|
| 蛋白质分子量测定过程中的酸效应 | |
| 引用本文： | 石磊,季怡萍,邢俊鹏,刘文祥,刘志强,刘淑莹. 蛋白质分子量测定过程中的酸效应[J]. 分析化学, 2002, 30(8): 938-941 |
| 作者姓名： | 石磊 季怡萍 邢俊鹏 刘文祥 刘志强 刘淑莹 |
| 作者单位： | 1. 中国科学院长春应用化学研究所,长春,130022;吉林大学公共卫生学院,长春,130021 2. 中国科学院长春应用化学研究所,长春,130022 3. 吉林大学公共卫生学院,长春,130021 |
| 摘 要： | 在基质辅助激光解吸电离飞行时间质谱（MALDI-TOF-MS）和电喷雾质谱（ESI-MS）测定蛋白质分子分子量的过程中,适当提高样品的酸度,可提高分析测试的灵敏度。在选定最佳样品分子浓度的基础上,通过适当加入三氟乙酸（TFA）来调整测试样品的酸度,准确测定了标准蛋白质-溶菌酶（lysozyme）的分子量,并且对蛋白质分子在“软电离”质谱中,受酸效应的影响进行了初步探讨。 |
| 关 键 词： | 蛋白质 分子量测定 酸效应 基质辅助激光解吸电离飞行时间质谱 电喷雾质谱 溶菌酶 |
Acid Effects on Protein Molecular Weight Determination |
|
| Shi Lei ,,Ji Yiping ,Xing Junpeng ,Liu Wenxiang ,Liu Zhiqiang ,Liu Shuying. Acid Effects on Protein Molecular Weight Determination[J]. Chinese Journal of Analytical Chemistry, 2002, 30(8): 938-941 | |
| Authors: | Shi Lei Ji Yiping Xing Junpeng Liu Wenxiang Liu Zhiqiang Liu Shuying |
| Affiliation: | Shi Lei 1,2,Ji Yiping 1,Xing Junpeng 1,Liu Wenxiang 2,Liu Zhiqiang 1,Liu Shuying *1 1 |
| Abstract: | The acid effects of some proteins on measuring their molecular weights were studied using matrix assisted laser desorption/ionization time of flight mass spectrometry (MALDI TOF MS) and electrospray ionization mass spectrometry (ESI MS). It was found that the signal intensity was enhanced through adjusting the acid concentration in some protein samples. In this paper, both MALDI MS and ESI MS was applied to examine the molecular weights of several standard proteins. And the proper acid concentration was detected in these spectra. In the meanwhile, it demonstrates that some associations of proteins in solution can be preserved into the gas phase and observed by the "soft ionization" mass spectrometry. |
| Keywords: | Matrix assisted laser desorption/ionization time of flight mass spectromery electrospray ionization mass spectrometry bovine serum albumin lysozyme trypsinogen |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |