Effects of stationary phase structure on retention and selectivity in reversed-phase liquid chromatography of flavonoid compounds with methanol as organic modifier |
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| Authors: | Y. D. Kahie C. Pietrogrande M. I. Medina Mendez P. Reschiglian F. Dondi |
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| Affiliation: | (1) Department of Analytical Chemistry, Somali National University, P.O. Box 1081, Mogadishu, Somalia;(2) Department of Chemistry, Analytical Chemistry Laboratory, Via L. Borsari 46, I-44100 Ferrara, Italy;(3) Facultad de Quimicas, Departmento de Quimica Analitica, Avda de las Ciencias S/N, Santiago de Compostela, Spain |
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| Abstract: | Summary Retention of an extended set of flavonoid compounds by octyl, octadecyl, phenyl and cyanopropyl-bonded, reversed-phase columns, with methanol, as the organic mobile phase modifier and acetic acid as the acid modifier is reported. Solvent strengths and useful ranges for both isocratic and gradient elution are determined. Relative retention is found to be independent of methanol volume fraction in the mobile phase for all the columns examined. Correlations between retention on different columns, specific selectivity effects and their dependence on molecular structure are analyzed. Practical applications for separation and identification are discussed. |
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| Keywords: | Column liquid chromatography Reversed-phase systems Octyl, octadecyl, phenyl and cyano-bonded phases Flavonoids Structure-retention relationships |
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