One-step immobilization of alkanethiol/glycolipid vesicles onto gold electrode: amperometric detection of Concanavalin A |
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Authors: | Guo Caixin Boullanger Paul Jiang Long Liu Tao |
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Institution: | Key Laboratory of Colloid and Interface Science, Center for Molecular Science, Institute of Chemistry, Chinese Academy of Science, Beijing, PR China. |
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Abstract: | Functionalized vesicles composed of glycolipid and alkanethiol lipids have been immobilized onto gold surface through one-step self-assembly to construct an electrochemical biosensor for Concanavalin A (Con A) detection. Incorporation of alkanethiol lipid molecules into the vesicles allows for firm attachment of the vesicles onto a gold surface to form a sensing interface. At the same time, the introduction of alkanethiol lipid avoids cumbersome organic syntheses of sulfur-containing compound, making the biosensor greater applied prospect. Through the recognition of Con A by glycolipid which was immobilized on the surface of electrode, a decrease of electrochemical signal was observed. This decrease was restored when the electrode was immersed in a stronger binding solution such as glucose. The repeated usability of the novel sensor is excellent. |
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