A novel impedance immunosensor based on O-phenylenediamine modified gold electrode to analyze abscisic acid |
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| Authors: | Qian Li Ruo Zhong Wang Zhi Gang Huang He Song Li Lang Tao Xiao |
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| Institution: | Hunan Provincial Key Laboratory of Phytohormones and Growth Development, Hunan Provincial Key Laboratory for Crop Germplasm Innovation and Utilization, Hunan Agricultural University, Changsha 410128, China |
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| Abstract: | An impedance immunosensor based on O-phenylenediamine modified gold electrode for the determination of phytohormone abscisic acid(ABA)was proposed.The operating pH,absorption time,absorption temperature and concentration of anti-ABA antibody were investigated to optimize the analytical performance.The calibration curve for the determination of ABA was obtained from this impedance immunosensor under optimal conditions.The results showed that the detection limit at about 1 ng/mL in the range of 10-5000 ng/mL. |
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| Keywords: | O-Phenylenediamine Abscisic acid Immunosensor |
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