Abstract: | The Glucose amount of human blood is very vital because in higher levels than allowed value the corporal biological system was hampered. Therefore, in this study, the Cu2O was deposited on the reduced Graphene oxide (RGO) by polydopamin (PDA) as linker. The new RGO‐PDA‐Cu2O nanocomposite was deposited on the glassy carbon electrode (GCE) surface after its characterization by UV–Visible, fourier transform infrared (FT‐IR), X‐ray diffraction (XRD), Energy‐dispersive X‐ray (EDX), transmission electron microscopy (TEM) and field emission scanning electron microscopy (FESEM) techniques. The electroanalysis of the new electrode was investigated by the cyclic voltammetry (CV), differential pulse voltammetry (DPV) and electrochemical impedance spectroscopy (EIS) methods. The obtained detection limit of glucose (Glu) showed that the deposited GCE by RGO‐PDA‐Cu2O nanocomposite has a high potential for its diagnosis. In addition, this electrode was applied to the Glu detection as biosensor in real samples in order to utilize in commercial applications. |