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Size-selective separation of gold nanoparticles using isoelectric focusing electrophoresis (IEF)
Authors:Arnaud Isabelle  Abid Jean-Pierre  Roussel Christophe  Girault Hubert H
Institution:Ecole Polytechnique Fudurale de Lausanne, Laboratoire d'Electrochimie Physique et Analytique, 1015-Lausanne, Switzerland.
Abstract:Isoelectric focusing in a polyacrylamide pH gradient gel is used to analyze the size distribution of gold nanoparticles synthesized by a chemical route with mercaptosuccinic acid as a ligand. The isoelectric point of the nanoparticles is shown to be size dependent, allowing fractionation by electrophoresis. Each fraction has a narrow size distribution with a standard deviation lower than 0.4 nm.
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