Electrochemical detection in a microfluidic device of oxidative stress generated by macrophage cells |
| |
Authors: | Amatore Christian Arbault Stéphane Chen Yong Crozatier Cécile Tapsoba Issa |
| |
Institution: | Ecole Normale Supérieure, Département de Chimie, UMR CNRS-ENS-UPMC 8640 "PASTEUR", 24 rue Lhomond, Paris Cedex 05, 75231, France. |
| |
Abstract: | The release of reactive oxygen species (ROS) or reactive nitrogen species (RNS), i.e., the initial phase of oxidative stress, by macrophage cells has been studied by electrochemistry within a microfluidic device. Macrophages were first cultured into a detection chamber containing the three electrodes system and were subsequently stimulated by the microinjection of a calcium ionophore (A23187). Their production of ROS and RNS was then measured by amperometry at the surface of a platinized microelectrode. The fabricated microfluidic device provides an accurate measurement of oxidative release kinetics with an excellent reproducibility. We believe that such a method is simple and versatile for a number of advanced applications based on the detection of biological processes of secretion by a few or even a single living cell. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|