The analysis of thyroxine in human serum by an 'exact matching' isotope dilution method with liquid chromatography/tandem mass spectrometry |
| |
Authors: | Hopley Chris J Stokes Peter Webb Kenneth S Baynham Michael |
| |
Institution: | Laboratory of the Government Chemist (LGC Limited), Queens Road, Teddington, Middlesex TW11 0LY, UK. |
| |
Abstract: | A method for the analysis of thyroxine in human serum, utilising 'exact matching' isotope dilution mass spectrometry (IDMS) in combination with liquid chromatography/tandem mass spectrometry (LC/MS/MS), has been developed with a limit of quantification of 0.5 ng g(-1) of thyroxine in human serum. The extraction and clean-up of thyroxine from serum involves an efficient protein-precipitation stage followed by a solid-phase extraction procedure to produce an extract essentially free from interfering compounds. The method is reproducible, with expanded uncertainties of less than 2%, and is relatively fast to perform. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|