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| 阳离子选择性耗尽进样-胶束扫集法测定尿液中的麻黄碱和可待因 | |
| 引用本文： | 李斯光,李海燕,蔡卓,程昊,李利军.阳离子选择性耗尽进样-胶束扫集法测定尿液中的麻黄碱和可待因[J].分析化学,2009,37(8). |
| 作者姓名： | 李斯光 李海燕 蔡卓 程昊 李利军 |
| 作者单位： | 1. 广东科贸职业学院,广州,510430;广西大学化学与化学工程学院,南宁,530004 2. 中山大学中山医学院,广州,510080 3. 广西大学化学与化学工程学院,南宁,530004 4. 广西工学院生物与化学工程系,柳州,545006 |
| 基金项目： | 广西自然科学基金，'5广东科贸学院院级课题 |
| 摘 要： | 联用选择性耗尽进样和胶束扫集两种在线富集技术,建立了尿样中麻黄碱和可待因含量测定的灵敏方法,并通过日内、日间、柱间实验考察了方法的稳定性.胶束扫集电动色谱缓冲体系为80 mmol/L 十二烷基磺酸钠(SDS)-20 mmol/L NaH2PO4(pH 2.20)-18%乙腈(V/V),分离电压-20 kV,进样电压10 kV,进样时间150 s,测量波长200 nm.同时讨论了pH值、SDS浓度、选择性耗尽进样萃取液电导、进样电压、进样时间和进水长度等对分离效果的影响.结果显示,方法富集功能很强,对麻黄碱和可待因含的富集倍数分别达5800和2490以上.在优化条件下,方法线性关系良好(r=0.9999),麻黄碱和可待因的线性范围分别为0.500～16.0 μg/L和2.00～48.0 μg/L,检出限分别为0.10和0.80 μg/L.方法稳定性良好,日内、日间和柱间的RSD分别为2.6%,5.9%和6.6%.应用于实际尿样分析,回收率在96.8%～106%之间,RSD≤4.7%,结果比较满意. |
| 关 键 词： | 选择性耗尽进样 胶束扫集电动色谱 麻黄碱 可待因 人尿 |
Determination of Ephedrine and Codeine in Human Urine by Cation-Selective Exhaustive Injection and Sweeping Micellar Electrokinetic Chromatography |
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| LI Si-Guang,LI Hai-Yan,CAI Zhuo,CHENG Hao,LI Li-Jun.Determination of Ephedrine and Codeine in Human Urine by Cation-Selective Exhaustive Injection and Sweeping Micellar Electrokinetic Chromatography[J].Chinese Journal of Analytical Chemistry,2009,37(8). | |
| Authors: | LI Si-Guang LI Hai-Yan CAI Zhuo CHENG Hao LI Li-Jun |
| Institution: | LI Si-Guang1,3,LI Hai-Yan3,CAI Zhuo4,CHENG Hao2,LI Li-Jun2 1(Guangdong Vocational College of Science and Trade,Guangzhou 510430)2(Department of Biological and Chemical Engineering,Guangxi University of Technology,Liuzhou 545006)3(Zhongshan School of Medicine,Sun-Yat-Sen Vniversity,Guangzhou 510080)4(College of Chemistry and Chemical Engineering,Guangxi University,Nanning 530004) |
| Abstract: | Two online preconcentration techniques including cation-selective exhaustive injection(CSEI),and sweeping micellar electrokinetic chromatography(sweep-MEKC)were used in determination of Ephedrine(Ep)and Codeine(Code)in human urine.Stability was also evaluated by intra-day,inter-day and inter-column experiment.The separation buffer contained 80 mmol/L SDS,20mmol/L NaH2PO4(pH=2.20),and 18% ACN(V/V).The sample was injected at 10 kV for 150 s,separated at-20 kV,and detected at 200 nm.Effects of pH,SDS concentra... |
| Keywords: | Cation-selective exhaustive injection sweeping micellar electrokinetic chromatography ephedrine codeine urine |
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