Determination of phylloquinone (vitamin K 1) by high performance liquid chromatography with UV detection and with particle beam-mass spectrometry |
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Authors: | M Careri A Mangia P Manini and N Taboni |
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Institution: | (1) Dipartimento di Chimica Generale ed Inorganica, Chimica Analitica, Chimica Fisica, Università degli Studi di Parma, I-43100 Parma, Italy |
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Abstract: | A reversed-phase PB LC-MS method was used for the determination and unequivocal identification of vitamin K1 in some vegetable samples. The method was also applied to the identification of other constituents in the food analyzed. LC-MS experiments were carried out in both EI and negative-ion CI modes. Preliminary sensitivity studies on vitamin K1 showed a detection limit of 2 ng (signal-to-noise ratio=3) in negative-ion operation and in single-ion monitoring at m/z 450. Quantitative results obtained with LC-MS for the extracts of four vegetables were compared with those of LC-UV analyses performed at 247 nm. |
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