Extraction and characterisation of anti-oestradiol immunoglobulins from human serum by high-performance liquid chromatography |
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| Authors: | O. Pepin N. Thuaud B. Sebille L. Edouard N. Lemort J. -L. Beaumont |
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| Affiliation: | (1) Laboratoire de Physico Chimie des Biopolymères, Institut de Recherches sur les Maladies Vasculaires, Université de Paris-Val de Marne, Avenue du Générale de Gaulle, F-94010 Crèteil, France;(2) Unite INSERM U32, Institut de Recherches sur les Maladies Vasculaires, Hopital Henri Mondor, 51, Avenue du Maréchal de Lattre de Tassigny, F-94010 Créteil, France |
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| Abstract: | Summary Immunoglobulins have been extracted from human and rabbit serum by size exclusion high performance liquid chromatography. In the case of rabbit immunized against ethinyl-oestradiol and woman having arterial thrombosis in the course of oral contraceptive use, the extracted immunoglobulins present a very high affinity for 17  -oestradiol sulphate. The measurement of affinity to this compound of different extracted immunoglobulins allows a quantitative determination of these specific proteins. Comparative studies with radioimmunoassays are in good agreement.Presented at the  14th International Symposium on Chromatography London, September, 1982 |
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| Keywords: | Hormone-protein-binding Oral contraceptures Aqueous size exclusion high-performance liquid chromatography Immunoglobulins Proteins separation |
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