Specific N-terminal protein labelling: use of FMDV 3C pro protease and native chemical ligation |
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| Authors: | Busch Gillian K Tate Edward W Gaffney Piers R J Rosivatz Erika Woscholski Rudiger Leatherbarrow Robin J |
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| Institution: | Department of Chemistry and the Chemical Biology Centre, Imperial College London, South Kensington Campus, London, UK. |
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| Abstract: | We report an effective strategy for generating N-terminal cysteinyl proteins by proteolytic cleavage using the enzyme 3C pro, suitable for a wide range of applications via native chemical ligation. |
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