| Crystal structure of the complex of trichosanthin with NADPH at 0.172 nm resolution |
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| 作者姓名： | XIONG Jian-Ping XIA Zong-Xiang WANG YuShanghai Institute of Organic Chemistry Chinese Academy of Sciences Shanghai SS China |
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| 作者单位： | XIONG，Jian-Ping XIA，Zong-Xiang WANG，YuShanghai Institute of Organic Chemistry，Chinese Academy of Sciences，Shanghai S0003S，China |
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| 基金项目： | Project supported by the National Key Laboratory of Bio-organic and Natural Product Chemistry. |
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| 摘 要： | The orthorhombic crystal structure of the complex of trichosanthin with nicotinamide adenine dinucleotide phosphate has been determined by molecular replacement method using one of the molecules of the monoclinic crystal structure of trichosanthin at 0.27 nm resolution as the search model. The crystallographic refinement at 0.172 nm resolution led to a final R-factor of 17.4% with root-mean-square deviations of 0.0013 nm and 3.8 from the ideal bond lengths and bond angles, respectively. The quality of the structure, the polypeptide chain fold and the comparison of it with that of the monoclinic trichosanthin structure, the location of nicotinamide adenine dinucleotide phosphate, the active site structure as well as the solvent structure are described.
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Crystal structure of the complex of trichosanthin with NADPH at 0.172 nm resolution |
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| XIONG,Jian-Ping XIA,Zong-Xiang WANG,YuShanghai Institute of Organic Chemistry,Chinese Academy of Sciences,Shanghai SS,China.Crystal structure of the complex of trichosanthin with NADPH at 0.172 nm resolution[J].Chinese Journal of Chemistry,1997,15(3):265-277. |
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| Authors: | XIONG Jian-Ping XIA Zong-Xiang WANG Yu |
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| Institution: | XIONG,Jian-Ping XIA,Zong-Xiang WANG,YuShanghai Institute of Organic Chemistry,Chinese Academy of Sciences,Shanghai S0003S,China |
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| Abstract: | The orthorhombic crystal structure of the complex of trichosanthin with nicotinamide adenine dinucleotide phosphate has been determined by molecular replacement method using one of the molecules of the monoclinic crystal structure of trichosanthin at 0.27 nm resolution as the search model. The crystallographic refinement at 0.172 nm resolution led to a final R-factor of 17.4% with root-mean-square deviations of 0.0013 nm and 3.8 from the ideal bond lengths and bond angles, respectively. The quality of the structure, the polypeptide chain fold and the comparison of it with that of the monoclinic trichosanthin structure, the location of nicotinamide adenine dinucleotide phosphate, the active site structure as well as the solvent structure are described. |
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| Keywords: | Complex trichosanthin NADPH crystal structure high-resolution |
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