生物碱分子表面印迹聚合物材料的设计与制备及其分子识别特性

采用巯基/偶氮二异丁腈(AIBN)表面引发体系,实现了甲基丙烯酸缩水甘油酯(GMA)在微米级硅胶微粒表面的引发接枝聚合,制得接枝微粒PGMA/SiO2；然后使接枝大分子PGMA的环氧基团与间二氨基苯磺酸钠(SAS)分子中的对位氨基发生开环反应,将苯磺酸钠基团键合在接枝大分子侧链,制得苯磺酸盐功能化的接枝微粒SAS-PGMA/SiO2。 在对功能微粒SAS-PGMA/SiO2与苦参碱分子间的相互作用进行考察研究的基础上,以戊二醛为交联剂,实施了苦参碱分子的表面印迹,制备了苦参碱分子表面印迹材料MIP-SASP/SiO2。 实验结果表明,在近中性溶液中,功能接枝微粒SAS-PGMA/SiO2与苦参碱分子间存在静电相互作用,以此为基础所设计与制备的表面印迹材料MIP-SASP/SiO2对苦参碱分子具有特异的识别选择性与优良的结合亲和性。 相对于对照物金雀化碱而言,该印迹材料对苦参碱的识别选择性系数为10.7。

Design and Preparation of Alkaloid Surface-Imprinted Material and Its Molecular Recognition Characteristics

The surface-initiated graft-polymerization of glycidyl methacrylate(GMA) on micron-sized silica gel particles was first conducted with a surface-initiating system of thiol group/azodiisobutyronitrile(AIBN) to obtain PGMA-grafted SiO2 particles. Sulfonate groups were bonded onto the side chains of PGMA by ring-opening reaction between the epoxy groups of PGMA and sodium 2,4-diaminobenzene sulfonate(SAS), resulting in the formation of SAS-PGMA/SiO2 particles. The interaction between SAS-PGMA/SiO2 and matrine was examined. Surface-imprinting of the matrine molecule was performed with glutaraldehyde as the crosslinking agent to yield the matrine molecule surface-imprinted material MIP-SASP/SiO2. The experiment results show that in the nearly neutral solution, there is strong host-guest interaction between the functional grafted particles SAS-PGMA/SiO2 and matrine. The surface-imprinted MIP-SASP/SiO2 particles, have specific recognition selectivity and excellent binding affinity for matrine. Relative to the cytosine control, the coefficient of recognition selectivity of the imprinted material MIP-SASP/SiO2 for matrine reaches 10.7.